Liquid biopsy cell-free DNA (cfDNA) in human plasma provides access to molecular information about the pathological processes in individuals. cfDNA analysis is widely used for the assessment of fetus chromosomal aberrations, graft rejection, monitoring tumor dynamics, and targeted treatment (i.e, circulating tumor DNA). cfDNA originates from pathogenic or healthy organisms, the precise mechanism of cfDNA release is unclear. Upon cell death, the genome is fragmented into nucleosome-complex sizes (~150bp, ~300 bp, ~450 bp, about 150bp per Unit size).