- R&D
- Clinical Diagnosis
- News
- Company
Frequently Asked Questions
Below you will find answers to the most common guestions people may have on Quick Biology.
If you cannot find the answer you are looking for, please contact us via the contact form on Contact page. We'd be happy to help!
In single reads, only one end of the nucleic acid fragments is sequenced. In paired-end reads, the nucleic acid fragments are first sequenced from one end, and then from the other end. This allows generation of high quality, alignable sequence data. Depending on the application, paired-end reads usually do not overlap. Paired-end reads can be used to estimate the fragment size and the duplication level of the library. In addition, paired-end sequencing allows detection of genomic rearrangements and repetitive sequence elements, as well as gene fusions and novel transcripts.