siRNA Array plate design and layout

siRNA Array - Drug Target I, II and III

Plate Design and Layout:

siRNA array design plate image.png

siRNA Array targeted genes and pathways:

siRNA Array 1: Apoptosis, Cell cycle, Cytoskeleton, DNA damage, HDAC.

siRNA Target Gene Title   [Common Name] Pathway
1 BCL2 B-cell   CLL/lymphoma 2 Apoptosis
2 CASP3 caspase 3,   apoptosis-related cysteine peptidase
3 XIAP X-linked   inhibitor of apoptosis, E3 ubiquitin protein ligase
4 AURKB aurora   kinase B Cell cycle
5 CDK1 cyclin-dependent   kinase 1
6 CDK4 cyclin-dependent   kinase 4
7 MDM2 MDM2   proto-oncogene, E3 ubiquitin protein ligase
8 PLK1 polo-like   kinase 1
9 FNTA farnesyltransferase,   CAAX box, alpha
10 KIF11 kinesin family   member 11
11 RPS6KA3 ribosomal   protein S6 kinase, 90kDa, polypeptide 3
12 PTK2 protein   tyrosine kinase 2 [FAK] Cytoskeleton
13 ROCK1 Rho-associated,   coiled-coil containing protein kinase 1
14 MAPT microtubule-associated   protein tau
15 ATM ATM   serine/threonine kinase DNA Damage
16 CHEK1 checkpoint   kinase 1
17 PARP1 poly   (ADP-ribose) polymerase 1
18 TOP1 topoisomerase   (DNA) I
19 TOP2A topoisomerase   (DNA) II alpha
20 PRKDC protein kinase,   DNA-activated, catalytic polypeptide [DNAPK]
21 PPP1R15A protein   phosphatase 1, regulatory subunit 15A [GADD34]
22 Background   (without cells)
23 Scrambled   siRNA 
24 Fluorescent   siRNA

siRNA Array 2: Kinase signaling pathways (MEK-ERK, MTOR-S6, NF-κB, NRTK, PAK, PI3K-AKT, PRKC),NOTCH and TNFα signaling pathways.

siRNA Target Gene Title   [Common Name] Pathway
1 BRAF B-Raf   proto-oncogene, serine/threonine kinase MEK-ERK
2 MAPK8 mitogen-activated   protein kinase 8 [JNK1]
3 MAP2K1 mitogen-activated   protein kinase kinase 1 [MEK1]
4 NTRK1 neurotrophic   tyrosine kinase, receptor, type 1
5 MAPK1 mitogen-activated   protein kinase 1 [p38]
6 PPM1D protein   phosphatase, Mg2+/Mn2+ dependent, 1D
7 PTPN6 protein   tyrosine phosphatase, non-receptor type 6 [SHP1]
8 MTOR mechanistic   target of rapamycin (serine/threonine kinase)  MTOR-S6
9 RPS6KB1 ribosomal   protein S6 kinase, 70kDa, polypeptide 1 [p70 S6KA]
10 IKBKE inhibitor   of kappa light polypeptide gene enhancer in B-cells, kinase epsilon [IKKE] NF-KB
11 NFKB1 nuclear factor   of kappa light polypeptide gene enhancer in B-cells 1
12 APH1A APH1A gamma   secretase subunit NOTCH
13 PSENEN presenilin   enhancer gamma secretase subunit
14 ABL1 breakpoint   cluster region/ABL proto-oncogene 1, non-receptor tyrosine kinase NRTK
15 BMX BMX   non-receptor tyrosine kinase
16 ITK IL2-inducible   T-cell kinase
17 PAK1 p21   protein (Cdc42/Rac)-activated kinase 1 PAK
18 AKT1 v-akt   murine thymoma viral oncogene homolog 1  PI3K-AKT
19 PDK1 3-phosphoinositide   dependent protein kinase 1
20 PIK3CA phosphatidylinositol-4,5-bisphosphate   3-kinase, catalytic subunit alpha
21 PRKCA protein   kinase C, alpha PRKC
22 Background   (without cells)
23 Scrambled   siRNA 
24 Fluorescent   siRNA

siRNA Array 3: Rac GTPases, Retinoic acid X family, RTK, SMO,Protein modifications, Metabolism.

siRNA Target Gene Title   [Common Name] Pathway
1 ARFGAP1 ADP-ribosylation   factor GTPase activating protein 1 Rac GTPases
2 RAC1 ras-related C3   botulinum toxin substrate 1 (rho family, small GTP binding protein Rac1)
3 RXRA retinoid   X receptor, alpha Retinioic acid X   family
4 ABL1 ABL   proto-oncogene 1, non-receptor tyrosine kinase RTK
5 ALK anaplastic   lymphoma receptor tyrosine kinase
6 AURKA aurora kinase A
7 BTK Bruton   agammaglobulinemia tyrosine kinase
8 EGFR epidermal   growth factor receptor
9 FGFR1 fibroblast   growth factor receptor 1
10 FLT3 fms-related   tyrosine kinase 3
11 IGF1R insulin-like   growth factor 1 receptor
12 MET MET   proto-oncogene, receptor tyrosine kinase
13 SRC SRC   proto-oncogene, non-receptor tyrosine kinase 
14 SYK spleen tyrosine   kinase
15 KDR kinase insert   domain receptor [VEGFR2]
16 SMO smoothened,   frizzled class receptor SMO
17 HSPA4 heat   shock 70kDa protein 4 [HSP70] Protein modification
18 HSP90AA1 heat shock   protein 90kDa alpha (cytosolic), class A member 1 [HSP90]
19 PSMC5 proteasome 26S   subunit, ATPase 5
20 P4HA1 prolyl   4-hydroxylase, alpha polypeptide I
21 PRKAA1 protein   kinase, AMP-activated, alpha 1 catalytic subunit [AMPK] Metabolism
22 Background   (without cells)
23 Scrambled   siRNA 
24 Fluorescent   siRNA