RNA Sequencing Service (RNA-seq)

RNA sequencing (RNA-Seq) has revolutionized the exploration of the transcriptome.  Advances in the RNA sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the RNA. As a professional RNA-Seq service provider,  Quick Biology is able to provide a fast turnaround time (10-20 business days) and a very competitive price for RNA-seq.

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Quick Biology RNA-seq Data Analysis Workflow:


Sample requirements

•  Total RNA amount: ≥ 1 ug for normal input; minimum 100 ng RNA input. For single-cell RNA, 10 pg to 10 ng of purified total RNA.

•  RIN value ≥ 6.5 for plants and fungi, RIN value ≥ 7.0 for human and animals

•  Purity: OD260/280 = 1.8-2.2, OD260/230 ≥ 2.0 without degradation or DNA contamination

Bioinformatic Service for RNA-seq

Standard analysis

        a.Raw data QC and clean up

        b.Alignment to a reference with mapping statistics

        c.Gene and transcript-based quantitation, TPM/RPKM/FPKM-based quantitation, Raw hit count-based quantitation

        d.Differentially Expressed Gene


        f.Gene Ontology

        g.Pathway Analysis

        h.Gene interaction network

        i.  Final project report with analysis methods and results

Advanced analysis

        j.Alternative pre-mRNA splicing

        k.RNA editing

        l.Antisense transcript 

        m.Novel transcripts

        n.Fusion genes/transcript detection 

Turnaround time

About 3-4 weeks, depends on the quality of submitted samples, one additional week for data analysis.