Obtain whole genome sequences with comprehensive genome coverage and exceptional sequence fidelity from single cells or low input gDNA.
The proliferation of technologies that enabled the analysis of single, isolated cells has transformed research in neuroscience, cancer, reproduction, and more. With technological breakthroughs in single cell isolation, whole genome amplification (WGA) and NGS library preparation, experiments using single cells are now possible – opening a wealth of exciting new insights for you to discover. However, challenges still exist. In particular, methods for the unbiased and complete amplification of a single genome and for the efficient conversion of that amplified DNA into a sequencer-compatible library face several technical limitations including incomplete amplification, the introduction of PCR errors, GC-bias and locus or allelic drop-out.
With these considerations in mind, Quick Biology offers optimized solutions for your research with the new low input / single cell whole genome sequencing (WGS) service.
Quick Biology’s single cell / low-input WGS service (scWGS) delivers:
· High sensitivity: Typical yield is greater than 5 µg when starting with 1 ng of genomic DNA. Whole genome amplification from as low as 6 pg DNA (5 human cells).
· Reduced amplification bias and no primer artifacts: Primer-free amplification method utilizes a combination of TthPrimPol Primase (to synthesize primers) and Phi29 DNA polymerase.
· Obtain higher genome coverage and greater sequence fidelity.
Quick Biology scWGS workflow:
We follow a complete WTA workflow from cell lysis, cDNA synthesis, highly uniform amplification across the entire genome to the final Illumina Sequencing in an optimized protocol.
· 1–1000 intact cells from many species (e.g., human, mouse, rat, bacteria, sorted cells, tissue culture cells, cells picked under the microscope or microdissected cells from frozen tissue).
· 50 pg – 1 ng of purified gDNA, free of any contaminants.
About 4 weeks, depends on the quality of submitted samples, one additional week for data analysis.