Introduction
RNA sequencing (RNA-Seq) has revolutionized the exploration of the transcriptome. Advances in the RNA sequencing workflow, from sample preparation through data analysis, enable rapid profiling and deep investigation of the RNA. As a professional RNA-Seq service provider, Quick Biology is able to provide a fast turnaround time (10-20 business days) and a very competitive price for RNA-seq.
Workflow
Sample Requirment
Total RNA amount: ≥ 1 ug for normal input; minimum 100 ng RNA input. For single-cell RNA, 10 pg to 10 ng of purified total RNA.
RIN value ≥ 6.5 for plants and fungi, RIN value ≥ 7.0 for human and animals
Purity: OD260/280 = 1.8-2.2, OD260/230 ≥ 2.0 without degradation or DNA contamination
Data Analysis
Standard analysis
a.Raw data QC and clean up
b.Alignment to a reference with mapping statistics
c.Gene and transcript-based quantitation, TPM/RPKM/FPKM-based quantitation, Raw hit count-based quantitation
d.Differentially Expressed Gene
e.Clustering
f.Gene Ontology
g.Pathway Analysis
h.Gene interaction network
i. Final project report with analysis methods and results
Advanced analysis
j.Alternative pre-mRNA splicing
k.RNA editing
l.Antisense transcript
m.Novel transcripts
n.Fusion genes/transcript detection
Turnaround Time
About 3-4 weeks, depends on the quality of submitted samples, one additional week for data analysis.